fe mesh Search Results


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Chem Impex International protoporphyrin ix
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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Vorwerk Elektrowerke GmbH Co KG fe mesh nodes
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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Simmetrix Inc fe mesh generation
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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ANSYS inc this pre- and postprocessor offers a parametric definition of geometry and the fe mesh
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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ANSYS inc 3d fe mesh
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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COMSOL Inc fe mesh exported by scan ip
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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COMSOL Inc fe mesh
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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ANSYS inc fe mesh of the building
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
Fe Mesh Of The Building, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ANSYS inc mesoscopic fe wire mesh model
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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COMSOL Inc finite element (fe) mesh of the lung
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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XYZ Scientific Applications Inc fe mesh preprocessor truegrid v. 2.3
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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XYZ Scientific Applications Inc fe mesh pre-processor truegrid v. 3.2
Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds <t>protoporphyrin</t> IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.
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Image Search Results


Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds protoporphyrin IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.

Journal: The Journal of Biological Chemistry

Article Title: High-throughput screening yields several small-molecule inhibitors of repeat-associated non-AUG translation

doi: 10.1074/jbc.RA119.009951

Figure Lengend Snippet: Small molecules inhibit CGG RAN translation in multiple reading frames. A, structures of compounds used in B–H. B–F, compounds identified from screen as RAN translation inhibitors were independently reassessed for their activity in RRL in vitro translation assays. The indicated compounds were added at increasing doses to 30% RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs, and luminescence was measured relative to vehicle (DMSO) treatment. G and H, candidate compounds protoporphyrin IX and TMPyP4 were added at increasing concentrations to RRL reactions with AUG, +1CGGx100, or +2CGGx100-NLuc reporter mRNAs. Luminescence was measured relative to vehicle (DMSO) treatment. For all graphs, n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test.

Article Snippet: Independent validation of hits in RRL system The following compounds were purchased through Sigma Marketsite: cephalothin sodium (Sigma–Aldrich, C4520), rufloxacin HCl (Vitas-M Laboratory, Ltd., STK711124), rofecoxib (Sigma–Aldrich, MFCD00935806), alfuzosin HCl (Sigma–Aldrich, A0232), olanzapine (Sigma–Aldrich, O1141), protoporphyrin IX (Chem-Impex Int, Inc., 21661), pefloxacine mesylate (Selleck Chemicals, S1855), reserpine (Sigma–Aldrich, MFCD00005091), isoxicam (Sigma–Aldrich, MFCD000079374), phenazopyridine HCl (Sigma–Aldrich, MFCD00035347), parbendazole (Sigma–Aldrich, MFCD018910864), balsalazide disodium salt dihydrate (Key Organics/BIONET, KS-5216), efavirenz (Sigma–Aldrich, SML0536), oxiconazole nitrate (Key Organics/BIONET, KS-5288), sulfamethazine sodium salt (Sigma, S5637-25G), kenpaullone (Adooq Bioscience, A11220), propidium iodide (Sigma, P4170), BIX01294 HCl hydrate (Cayman Chemical, 13124), anthralin (Key Organics/BIONET, KS-5183), CP-31398 dihydrochloride hydrate (Tocris Bioscience, 3023), X80 (Sigma, X3629), amlexanox (Sigma, 68302-57-8).

Techniques: Activity Assay, In Vitro, Comparison

Small molecules inhibit C9RAN translation in all three reading frames. BIX01294 (A), anthralin (B), and protoporphyrin IX (C) were added at increasing concentrations to 30% RRL in vitro translation reactions with AUG-NLuc or GGGGCCx70 reporter mRNAs for all three reading frames, and luminescence was measured relative to vehicle (DMSO) control. D–F, selective inhibition of RAN translation of GGGGCCx70 and CGGx100 NLuc reporter mRNAs, relative to AUG-NLuc control, was confirmed by Western blotting against a C-terminal FLAG tag for the glycine-alanine (GA) and FMRPolyG (+1CGG) products, following incubation with BIX01294, anthralin, or protoporphyrin IX at the indicated doses. GAPDH was used as a loading control. For A–C, all graphs represent mean of n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test. M, mock; GA, glycine-alanine; GP, glycine-proline; GR, glycine-arginine.

Journal: The Journal of Biological Chemistry

Article Title: High-throughput screening yields several small-molecule inhibitors of repeat-associated non-AUG translation

doi: 10.1074/jbc.RA119.009951

Figure Lengend Snippet: Small molecules inhibit C9RAN translation in all three reading frames. BIX01294 (A), anthralin (B), and protoporphyrin IX (C) were added at increasing concentrations to 30% RRL in vitro translation reactions with AUG-NLuc or GGGGCCx70 reporter mRNAs for all three reading frames, and luminescence was measured relative to vehicle (DMSO) control. D–F, selective inhibition of RAN translation of GGGGCCx70 and CGGx100 NLuc reporter mRNAs, relative to AUG-NLuc control, was confirmed by Western blotting against a C-terminal FLAG tag for the glycine-alanine (GA) and FMRPolyG (+1CGG) products, following incubation with BIX01294, anthralin, or protoporphyrin IX at the indicated doses. GAPDH was used as a loading control. For A–C, all graphs represent mean of n = 3; error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test. M, mock; GA, glycine-alanine; GP, glycine-proline; GR, glycine-arginine.

Article Snippet: Independent validation of hits in RRL system The following compounds were purchased through Sigma Marketsite: cephalothin sodium (Sigma–Aldrich, C4520), rufloxacin HCl (Vitas-M Laboratory, Ltd., STK711124), rofecoxib (Sigma–Aldrich, MFCD00935806), alfuzosin HCl (Sigma–Aldrich, A0232), olanzapine (Sigma–Aldrich, O1141), protoporphyrin IX (Chem-Impex Int, Inc., 21661), pefloxacine mesylate (Selleck Chemicals, S1855), reserpine (Sigma–Aldrich, MFCD00005091), isoxicam (Sigma–Aldrich, MFCD000079374), phenazopyridine HCl (Sigma–Aldrich, MFCD00035347), parbendazole (Sigma–Aldrich, MFCD018910864), balsalazide disodium salt dihydrate (Key Organics/BIONET, KS-5216), efavirenz (Sigma–Aldrich, SML0536), oxiconazole nitrate (Key Organics/BIONET, KS-5288), sulfamethazine sodium salt (Sigma, S5637-25G), kenpaullone (Adooq Bioscience, A11220), propidium iodide (Sigma, P4170), BIX01294 HCl hydrate (Cayman Chemical, 13124), anthralin (Key Organics/BIONET, KS-5183), CP-31398 dihydrochloride hydrate (Tocris Bioscience, 3023), X80 (Sigma, X3629), amlexanox (Sigma, 68302-57-8).

Techniques: In Vitro, Inhibition, Western Blot, FLAG-tag, Incubation, Comparison

RAN translation inhibitors have varying effects on AUG-initiated repeat translation. A, schematic of NLuc reporter mRNAs used in 30% RRL in vitro translation assays, illustrating the position of the AUG start codons inserted upstream of the repeats, to drive translation in the indicated reading frames. The effect of anthralin (B), TMPyP4 (C), protoporphyrin IX (D), BIX01294 (E), and CP-31398 (F) on +1 and +2CGGx100 RAN translation, compared with AUG-initiated CGGx100 translation, measured in the presence of increasing concentrations of each small molecule. For B–F, values are relative to DMSO controls, and graphs represent the mean of n = 3. Error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test. 3xF, 3xFLAG tag.

Journal: The Journal of Biological Chemistry

Article Title: High-throughput screening yields several small-molecule inhibitors of repeat-associated non-AUG translation

doi: 10.1074/jbc.RA119.009951

Figure Lengend Snippet: RAN translation inhibitors have varying effects on AUG-initiated repeat translation. A, schematic of NLuc reporter mRNAs used in 30% RRL in vitro translation assays, illustrating the position of the AUG start codons inserted upstream of the repeats, to drive translation in the indicated reading frames. The effect of anthralin (B), TMPyP4 (C), protoporphyrin IX (D), BIX01294 (E), and CP-31398 (F) on +1 and +2CGGx100 RAN translation, compared with AUG-initiated CGGx100 translation, measured in the presence of increasing concentrations of each small molecule. For B–F, values are relative to DMSO controls, and graphs represent the mean of n = 3. Error bars, S.D. *, p < 0.05, two-way ANOVA with Dunnett's multiple-comparison test. 3xF, 3xFLAG tag.

Article Snippet: Independent validation of hits in RRL system The following compounds were purchased through Sigma Marketsite: cephalothin sodium (Sigma–Aldrich, C4520), rufloxacin HCl (Vitas-M Laboratory, Ltd., STK711124), rofecoxib (Sigma–Aldrich, MFCD00935806), alfuzosin HCl (Sigma–Aldrich, A0232), olanzapine (Sigma–Aldrich, O1141), protoporphyrin IX (Chem-Impex Int, Inc., 21661), pefloxacine mesylate (Selleck Chemicals, S1855), reserpine (Sigma–Aldrich, MFCD00005091), isoxicam (Sigma–Aldrich, MFCD000079374), phenazopyridine HCl (Sigma–Aldrich, MFCD00035347), parbendazole (Sigma–Aldrich, MFCD018910864), balsalazide disodium salt dihydrate (Key Organics/BIONET, KS-5216), efavirenz (Sigma–Aldrich, SML0536), oxiconazole nitrate (Key Organics/BIONET, KS-5288), sulfamethazine sodium salt (Sigma, S5637-25G), kenpaullone (Adooq Bioscience, A11220), propidium iodide (Sigma, P4170), BIX01294 HCl hydrate (Cayman Chemical, 13124), anthralin (Key Organics/BIONET, KS-5183), CP-31398 dihydrochloride hydrate (Tocris Bioscience, 3023), X80 (Sigma, X3629), amlexanox (Sigma, 68302-57-8).

Techniques: In Vitro, Comparison